Fig. 11. Effect of glutamate (E) to lysine (K) substitutions at positions 145 (E145K) and 152 (E152K) on the voltage dependence of yeast VDAC. The steepness of the voltage dependence was measured in the presence of 5.75 x 10-2 mg/ml dextran sulfate (500kDa), a value that magnifies the steepness of the voltage dependence by a factor of 10 (see also Fig. 15). The two bars indicate the voltage dependence of each of the two gating processes. This is a replotting of the data of Zizi et al., 1995.