|An Arabidopsis 2010 project to characterize pre-mRNA splicing signals
Existing genefinders use information about splice sites and differences between exons and introns, but do not explicitly incorporate information about sequences known as splicing enhancers, which promote incorporation of exons into mRNA (an exception is ExonScan). This is a project to characterize exonic splicing enhancers in Arabidopsis. Although splicing enhancers likely function in splice site selection in many plant genes, and contribute to the regulation of alternative splicing, plant splicing enhancers have not yet been described in detail. Computational analysis of a database of Arabidopsis exons and introns has been used to identify candidate splicing enhancer sequences. The role of these sequences, and sequences from genes that are known to be alternatively spliced, are being tested in transgenic Arabidopsis using a splicing reporter construct. The activity of these enhancers has been examined in transgenes that depend on exon inclusion for expression.
This is NSF Award#0114792 - Arabidopsis 2010: Pre-mRNA Splicing Signals in Arabidopsis
PI,Stephen M. Mount, Ph.D., Associate Professor, Dept. of Cell Biology and Molecular Genetics, University of Maryland. (email: email@example.com)
co-PI,Caren Chang , Ph.D., Associate Professor, Dept. of Cell Biology and Molecular Genetics, University of Maryland.
co-PI,Steven Salzberg, Ph.D., Director, Center for Bioinformatics and Computational Biology , University of Maryland and Professor, Dept. of Computer Science, University of Maryland