Ph.D. Biochemistry, Weizmann Institute, 1982

Professor

Center for Advanced Research in Biotechnology

9600 Gudelsky Dr.

Rockville, MD 20850 USA

Phone: (301)-738-6245

E-mail: osnat@carb.nist.gov

Research: Protein Crystallography.

• Beta-lactame
• Troponin C
• PTS proteins: HPr and glucose permease IIA-domain
• Pyruvate phosphate dikinase
• S-lectin

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Protein Crystallography:

1. O. Herzberg and J. Moult, "Molecular Basis for Bacterial Resistance to Beta-Lactam Antibiotics: Crystal Structure of Beta-Lactamase from Staphylococcus Aureus PC1 at 2.5Å resolution", Science, 236, 694-701 (1987).
2. O. Herzberg, "Refined Crystal Structure of Beta-Lactamase from Staphylococcus aureus PC1 at 2.0Å resolution", J. Mol. Biol. 217, 701-719 (1991).
3. O. Herzberg, G. Kapadia, B. Blanco, T.S. Smith & A.F.W. Coulson, "Structural basis for the Inactivation of the P54 Mutant of Beta-lactamase from staphylococcus aureus PC1", Biochemistry 30, 9503-9509 (1991).
4. O. Herzberg & J. Moult, "Penicillin - binding and degrading enzymes", Current Opinion in Structural Biology, 1, 946-953 (1991).
5. C.C.H. Chen and O. Herzberg, "Inactivation of Beta-lactamase by clavulanate: trapped intermediates in cryo-cryocrystallographic studies", J. Mol. Biol. 224,1103-1113 (1992).
6. C.C.H. Chen, J. Rahil, R.F.Pratt & O. Herzberg, "Structure of a phosphonate-inhibited Beta-lactamase: An analog of the tetrahedral transition state/intermediate of Beta-lactam hydrolysis". J. Mol. Biol., 234, 165-178 (1993).
7. L. Zawadzke, T.J. Smith & O. Herzberg. "An engineered Staphylococcus aureus PC1 Beta-lactamase that hydrolyses third generation cephalosporins." Protein Engineering, 12, 1275-1285 (1995).
8. Nestor O. Concha, Beth A. Rasmussen, Karen Bush, and Osnat Herzberg. "Crystal structure of the wide-spectrum binuclear zinc beta-lactamase from Bacteroides fragilis" STRUCTURE 4, 823-836 (1996).
9. C.C.H. Chen, T.J. Smith, G. Kapadia, G., S. W sch, L.E. Zawadzke, A. Coulson, & O. Herzberg. "Structure and kinetics of the beta-lactamase mutants S70A and K73H from Staphylococcus Aureus PC1." Biochemistry 35, 12251-12258 (1996).
10. Zawadzke, L.E., Chen, C.C.H., Banerjee, S., Li, Z., W sch, S., Kapadia, K., Moult,J. & Herzberg, O. "Elimination of the hydrolytic water molecule in a class A beta-lactamase mutant: Crystal structure and kinetics." Biochemistry 35, 16475-16482 (1996).

1. O. Herzberg and M.N.G. James, "Structure of the Calcium Regulatory Muscle Protein Troponin-C at 2.8Å Resolution", Nature 313, 653-659 (1985).
2. O. Herzberg and M.N.G. James, "The Common Structural Framework of the Two Ca² +/Mg² + Binding Loops of Troponin-C and other Ca² + Binding Proteins", Biochemistry, 24, 5298-5302 (1985).
3. O. Herzberg, J. Moult and M.N.G. James, "A Model for the Ca² + Induced Conformational Transition of Troponin-C: a Trigger for Muscle Contraction", J. Biol. Chem., 261, 2638-2644 (1986).
4. O. Herzberg and M.N.G. James, "Refined Crystal Structure of Troponin C from Turkey Skeletal Muscle at 2.0Å Resolution", J. Mol. Biol., 203, 761-779 (1988).
5. O. Herzberg, J. Moult and M.N.G. James, "Structure of Troponin-C and its Implication for the Ca² + triggering of Muscle Contraction" in "Methods in Enzymology", vol. 139, pp. 610-632 (1987).
6. K. Fujimori, M. Sorenson, O. Herzberg, J. Moult & F.C. Reinach, "Probing the Ca² + -Induced Conformational Transition of Troponin C with Site-Directed Mutants", Nature, 344, 182-184 (1990).
7. J. Pearlstone, T. Borgford, M. Chandra, K. Oikawa, C.M. Kay, O. Herzberg, J. Moult, A. Herklotz, F.C. Reinach & L.B. Smillie "Construction and characterization of a spectral probe mutant of troponin C: Application to analyses of mutants with increased Ca² + affinity", Biochemistry 31, 6545-6553 (1991).
8. A.C.R Da Silva, A.H.B. De Araujo, O. Herzberg, J. Moult, M. Sorenson & F.C. Reinach, "Troponin C mutants with increased calcium affinity", Eur. J. Biochem. 213, 599-604 (1993).

1. D-I. Liao, G. Kapadia, P. Reddy, M.H.Jr. Saier, J. Reizer & O.Herzberg, "Structure of the IIA domain of the glucose permease of Bacillus subtilis at 2.2Å resolution", Biochemistry 30, 9583-9594 (1991).
2. D. Worthylake, N.D. Meadow, S. Roseman, D-I. Liao, O. Herzberg & S.J. Remington, " Three-dimensional structure of the Escherichia coli Phosphocarrier Protein III", Proc. Natl. Acad. Sci. USA 88, 10382-10386 (1991).
3. O. Herzberg, P. Reddy, M.H.Jr. Saier, J. Reizer & G. Kapadia, "Structure of the histidine-containing phosphocarrier protein HPr from Bacillus subtilis at 2.0Å resolution", Proc. Natl. Acad. Sci. USA. 89, 2499-2405 (1992).
4. O. Herzberg "An atomic model for protein-protein phosphoryl group transfer" J. Biol. Chem. 267, 24819-24823 (1992).
5. D-I. Liao & O. Herzberg. Refined structures of the active Ser83Cys and impaired Ser46Asp histidine-containing phosphocarrier proteins. Structure 2, 1203-1216 (1994).
6. U. Pieper, G. Kapadia, P-P. Zhu, A. Peterkofsky & O. Herzberg. Structural basis for the evolutionary divergence of mycoplasma from Gram-positive bacteria: the histidine-containing phosphocarrier protein. Structure 3, 781-790 (1995).
7. O. Hezberg & R. Klevit, "Unraveling a bacterial hexose transport pathway", Current Opinion in Structural Biology, 4, 814-822, (1994).

1. O. Herzberg, C.C.H. Chen, G. Kapadia, M. McGuire, L.J. Carroll, S.J. Noh & D. Dunaway-Mariano. "Swiveling-domain mechanism for enzymatic phosphotransfer between remote reaction sites." Proc. Natl. Acad. Sci. USA 93, 2652-2657 (1996).
2. Mcguire, M., Carrol, L., Yankie, L., Thrall, S.H., Dunaway-Mariano, D., Herzberg,O., Jayaram, B. & Haley, B. "Determination of the nucleotide binding site within Clostridium symbiosum pyruvate phosphate dikinase by photoaffinity labeling, site directed mutagenesis and structural analysis." Biochemistry 35, 8544-8552 (1996).

1. D-I. Liao, G. Kapadia, H. Ahmed, G.R. Vasta & O. Herzberg, "Structure of S-lectin, a developmentally regulated vertebrate Beta-galactoside binding protein". Proc. Natl. Acad. Sci. USA. 91, 1428-1432 (1994).
2. Y. Bourne, B. Bolgiano, D-I. Liao, G. Strecker, P. Cantau, O. Herzberg, T. Feizi & C. Cambillau, Crosslinking of mammalian lectin (galectin 1) by complex biantennary saccharides. Nature Structural Biology, 1, 863-869 (1994).

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