Methylene Blue is a homologue of Toluidine Blue O. This stain is used to visualize intracellular metachromatic granules.
- Smears are fixed with heat and allowed to cool.
- Stain with the methylene blue (homologue of toluidine blue O) solution two to seven minutes.
- Wash slide and blot dry.
Results: By this method, the intracellular metachromatic granules stain a ruby-red to black color; with the remainder of the cell staining a pale blue color.
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