BSCI 230;  Spring, 2001

Recitation Quiz #2

W. J. Higgins

 
 

1.


 

 
 

Use the axes above to plot the results of an experiment with an enzyme:
 

 
 
 
 

a.  Complete the labeling of the x and y axes by providing the units on the graph.
 

 
 
 
 

b.  Plot the expected data for a typical enzyme.  Label this curve as "A"
 

 
 
 
 

c.  Label Km and Vmax at their correct locations.  The units of Km are______________
 

 
 

The units of Vmax are _____________________________.
 

d.  Draw a plot of the data that would result if an allosteric inhibitor were added to the enzyme during the assay.  This inhibitor renders affected enzyme molecules inactive.  Label this plot "I".
 

 
 

e.  The allosteric inhibitor used in part d. above had what effect on the value of the enzyme's
 
 

Vmax ________________   Km ________________?
 

2.  ______________________________________:
 
 

 
Starting materials:___________________________________________

 
 
 

End Products:______________________________________________
 

 

Cellular location: _________________________________
 
 
 

What's the Point: ___________________________________________


 
 

3.  Draw your own rendition of the Chemiosmotic Synthesis of ATP.  Start with electrons liberated from glucose in glycolysis.