Instructor Steve Mount smount@umd.edu 301-405-6934
Meetings Tues. and Thur. 4:00-6:00 pm in Microbiology. We use the full two hours. The class will start on Tuesday, March 24, 2009.
Prerequisites:
Genetics and at least one advanced course in either Genetics, Biochemistry or Molecular Genetics at the undergraduate level. Those without these prerequisites are encouraged to take BSCI 410 before (or instead of) 688I. Most students in CBMG688I will be continuing from CBMG688F.
Readings:
Papers from the primary literature in molecular genetics are required reading. Papers and supplemental readings will be on available online; I will provide links through the syllabus. Details for 2009 are not yet determined, but the 2008 syllabus is available.
I like to address questions about the science in writing so that I can disseminate my answers to the entire class. Send your question to smount at umd.edu, in which case I will generally remove your name and post my answer so that everyone can see the question and its answer. Please tell me if are asking a question that you don't want to see posted. My plan is that questions and answers will posted on Blackboard (supplemented by web pages as appropriate), but I am open to suggestions about the most effective means of real-time communication with the class.
Textbooks:
| Primary text: Hartwell's "Genetics: From Genes to Genomes." 3rd edition. | |
 |
The third edition no longer includes sections on model organisms for genetic research, but these are available online at the McGraw-Hill web site. Registration is simple. |
About the second edition: The bookstore will not carry the second edition, and I will check page numbers against only a single edition (the third edition) when assigning readings. However, the second edition (or any other recent and comprehensive genetics text) should be adequate if you are prepared to use the index and other sources to find the information you need.
Additional books.
Many of these books (and others) are available online at NCBI (see www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Books).
These are the books that I consult when preparing my lectures.
- Hawley and Walker, Advanced Genetic Analysis: Finding Meaning in a Genome. Blackwell 2003.
An excellent source for advanced genetic concepts that are not presented elsewhere. I use it especially for meiosis.
- Strachan and Read. Human Molecular Genetics 3 (HMG3) Wiley. 2004. ISBN 978-0-8-1534182-6
This excellent book is a nice supplement for human genetics. I consult it regularly and will present some material from it.
The second edition is available online at NCBI. Some copies of the third edition are available in the book store.
- Alberts et al., Molecular Biology of the Cell. Garland. 2002 (4th edition).
This book is online at NCBI. A fifth edition is out this year, but I have not seen it yet.
- Griffiths et al., Introduction to Genetic Analysis. Freeman. Seventh Edition 2000.
Available online at NCBI. I use it especially for linkage and the Poisson distribution.
- Gibson and Muse, A Primer of Genome Science. Sinauer. 2004. (This is the Second edition; the first was 2002)
A source for Genomics.
- Mount, Bioinformatics: Sequence and Genome Analysis. CSHL Press. 2004. (This is the Second edition; the first was 2001)
Really the best book on Bioinformatics at this level. (The author is not a relative)
- Molecular Biology of the Gene. Watson et al. 2003 (5th edition).
- Weaver, Molecular Biology. WCB McGraw Hill. "2002" (2nd edition).
- Recombinant DNA: Genes and Genomes - A short course. 2007. Watson, Myers, Caudy and Witkowski.
Permission is required for this course. Keeping the class small is important for the paper presentations, but we have over 30 students this year. Only Ph.D. students will be granted permission to oversubscribe until the spring semester actually begins. After that, anyone can sign up. The policy is explained in detail at clfs.umd.edu/classroom/CBMG688I/permissions.html.
Coverage. Methods of genetic analysis and genomics. The primary species are genetic model organisms, the yeast Saccharomyces cerevisiae, the worm Caenorhabditis elegans, the plant Arabidopsis thaliana, the fruit fly Drosophila melanogaster, the mouse, and humans. For each of these species we will consider methods for the identification of informative mutations revealing new genes or new gene functions and methods for testing the expression of modified genes. Genomics, including applicable genomics resources and practical bioinformatics tools, will also be emphasized. We will bear in mind throughout the application of information from model organisms to organisms that are not among this select group.
Grading. Your grade will be determined as described in detail on another page. Most of your grade will be based on your reading of seven carefully selected papers from the genetics literature. The goal is to learn how to extract from a paper what claims are being made, how the results were obtained (i.e. which experiments were done), why the experiments were done the way they were, whether the data justify the conclusions and what caveats remain, why these experiments were done at all and why we should care.
On the date that a paper is to be discussed, come to class familiar with the paper, and with a copy of the paper in hand. Your familiarity with the papers will be judged by your participation in the discussion, including presentation of material (30% of your grade) and quizzes (40%). The remaining 30% of your grade will be based on homework.
Appointments outside of class: I will normally be available immediately after class. I am also happy to set up an appointment to meet with you at other times. Please send an email after consulting my online calendar.
modified: March 26, 2009