BSCI411, Plant Genetics & Molecular Biology
Midterm exam, March 15, 2001
Your Name:________________________ SS#_________________________
-: mutant in F1). How many complementation groups are there? How are these five mutations grouped into different complementation groups?
a1 a2 a3 a4 a5
a1 - + + + -
a2 - - + +
a3 - + +
a4 - +
a5 -
Whorls: 1 2 3 4
WT Sepal Petal Stamen Carpel
a mutant:
b muatnt:
c mutant:
bc double mutants:
c mutants carrying a 35S-B transgene:
seuss was originated in the Ler ecotype and was crossed into a Wild-type plant (Col ecotype). The F1 progeny of this cross is wild-type in phenotype but is heterozygous for seuss in genotype. He let the F1 plant self and then isolated DNA from 13 seuss mutant plants in F2. CZ1 primers were used to PCR-amplify the CZ1 locus from 13 individual seuss mutants. The PCR fragment was then diggested with EcoR1 and then run on a 1% agarose gel. The following is the image from the gel after electrophoreses:
Ler |
Col |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
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a. What type of a marker is CZ1? (RFLP? SSLP? CAPS? SNPs?).
b. What is the distance (in % recombination) between SEUSS and CZ1 ? Show your work.
ddA ddG ddC ddT (added to each sequencing reaction)
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Genomic DNA library vs. cDNA library
Microarray vs. DNA chip
Cis-regulatory elements vs. Trans-acting factors
CAPS vs. RFLP
Promoter vs Enhancer