BSCI 410 |
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Syllabus
Lectures are drafts until given, and perhaps a little afterwards (they can be revised at any time). If the lecture is in the Draft folder (the link is to private/Draft/LecNN.ppt), then you are definitely looking at a draft. In some cases, I will make no changes prior to class and only a few afterwards. Other lectures will be changed significantly, either before class, after class, or both. When I feel that the lecture is in its final form, then I will post it to the 2008 folder (private/2008/LecNN.ppt). If I haven't done anything at all yet, the lecture may still be in the 2007 folder (private/2007/LecNN.ppt), in which case it is simply last year's lecture.
Section I -- Genetic Information: replication, transmission and informatics
|
Date |
What |
Title |
Readings |
|
9-02 |
Lecture 1 |
Introduction -- What is Molecular Genetics? |
H Ch. 1;
Ch. 6, pp. 167-184 DNA as the genetic material |
|
9-04 |
DNA -- replication and segregation of chromosomes (mechanism, cell biology, mitosis) |
H Ch. 6 (184-191) DNA replication |
|
| 9-09 | Meiosis, the basis of Mendel's laws and probability for genetics |
H Ch. 2 Mendel's breakthrough H Ch. 4 The chromosome theory, esp. 93-103. Binomial and Poisson distributions (also see wikipedia, Griffiths) |
|
| 9-11 | Genetic linkage and maps | H Ch. 5 Linkage and Mapping, pp. 123-142 Chalfie 1997, mapping function in Griffiths Supplemental text Hawley and Walker Ch. 7 |
|
| 9-16 | Linkage continued: |
H 142-155 tetrad analysis; 759-762 Hardy-Weinberg; 105-113 Validation of the chromosome theory (also Bridges 1916) Hawley and Walker Ch. 7.3 & 7.4 |
|
| 9-18 | Genomics and Internet Resources; informatics |
H Ch. 10 Reconstructing the Genome |
Section II -- Molecular Biology and Gene Expression
|
Date |
What |
Title |
Readings |
| 9-23 | Escherichia coli as a model organism, molecular cloning Homework 1 is due. (Q&A) (answers) |
H Ch. 15 Genetic analysis in bacteria |
|
| 9-25 | Lecture 8 |
Labeling, hybridization, microarrays and PCR Homework 2 is due. (Q&A) (answers) Review for exam 1 |
H Ch. 9 DNA, 327-340. H. Ch. 11. |
| 10-02 | EXAM 1 (answers) |
Exam on lectures 1-6 (Q&A) | |
| 10-14 | Lecture 9 |
Mutagenesis, sequencing and DNA-protein interactions |
H Ch. 9 pp. 327-340; H. Ch. 11 Direct detection of phenotype web sites (for sequencing); Fig. 17.15 (p. 622) (Oct. '08 Nat. Biotech.; esp. Shendure and Ji) |
| 10-16 | Lecture 10 |
Genome organization: Genome structure and change | H. Ch. 10, pp. 366-384 H. Ch. 13, esp. pp. 508-515 H. Ch. 22, esp. 799-822 Nature Genome Gateway (incl. Chimp) Fitch 2000 on homology (PDF, PubMed) |
| 10-21 | Lecture 11 |
Repair and recombination begin transcription |
H. ch. 6, esp. Fig. 6-23 and chapter 7 Hartwell pp. 265-269. Alberts online (transcription and repair). |
| 10-23 | Lecture 12 | Transcription |
H. Ch. 17 Gene regulation in Prokaryotes; |
Section III -- Gene Expression and Genomics
|
Date |
What |
Title |
Readings |
| 10-28 | Regulation of transcription. | H Ch. 18,
especially 657-677 |
|
| 10-30 | Lecture 14 |
RNA Processing, translation, stability and localization |
H pp. 265-292 and 657-677 Nature: RNAi (esp. Matzke & Birchler) 2006 Nobel Prize in Medicine (Figure) |
| 11-04 | Genetic and physical maps -- positional cloning |
H Ch. 5; Ch. 10, esp. 351-366; Ch. 11 Human Molecular Genetics II (online) |
|
| 11-06 | EXAM 2 (answers) |
This exam will cover lectures 7-12 (review questions) (Q&A) |
|
| 11-11 | Lecture 16 |
LOD scores, complex traits and allelic association | H Ch. 5; Ch. 11; Ch. 20, esp. 773-781 Human Molecular Genetics II (online) Also see HAPMAP project in Nature (with commentary ) and HMGII (Chs. 11 and 12) |
| 11-13 | Lecture 17 |
Types of allele. | H. Chs. 3, 5, 8 (pp. 285-292), 11 (esp. 419-425), 20 (721-729) and 21 (773-781) |
Section IV -- Model Organisms and Genetic Analysis
|
Date |
What |
Title |
Readings |
| 11-18 | Lecture 18 |
Genome Projects and the relationships among species. An overview of genetic analysis in model organisms. | H. Chs. 22, 20, 10 (366-383) and 12 |
| 11-20 | Genetic analysis: Yeast (Saccharomyces cerevisiae), a model eukaryote. Homework 4 is due. (Q&A; answers: brief, full) |
Hartwell Ch. 20, Ref. A
|
|
| 11-25 | Lecture 20 |
Genetic analysis: model animals C. elegans and D. melanogaster |
Hartwell, Ch. 20, Ref. C and Ref. D |
| 12-02 | Lecture 21 |
Genetic analysis with inbred lines Arabidopsis thaliana (a model plant) Mus musculus (a model mammal) Review for exam 3 |
Hartwell Ref. B and Ref. E HMG II online Peters et al. 2007: "The mouse as a model for human biology: a resource guide for complex trait analysis." |
| 12-04 | EXAM 3 (answers) |
This exam will cover lectures 13-17 | |
| 12-9 | Genes in pathways: epistasis Radiolab on chimerism: March 14, 2008 |
Hartwell: epistasis, sex dtermination in flies, |
|
| 12-11 | Lecture 23 | Gene therapy, diagnostics, forensics, integration, comprehensive review. Homework 5 is due ( Q&A ; answers). |
Review in the context of modern genetics. The DNA Age - A Series of Articles From The New York Times |
| 12-15 8 am |
Final Exam (Q&A) (answers) |
Comprehensive final exam (one half will be on section IV, lectures 18-23, and one half will be comprehensive) | All readings! |
| 12-18 1-2 pm |
Exam viewing | 2109 HJP | You can see your exam (but not take it). |