BSCI 410 |
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Syllabus (2006)
Section I -- Introduction, Background and Review
|
Date |
What |
Title |
Readings |
|
8-31 |
Lecture 1 PowerPoint Links |
Introduction -- What is Molecular Genetics? |
H Ch. 1, pp. 1-12;
Ch. 6, pp. 155-169 DNA as the genetic material |
|
9-05 |
DNA -- replication and segregation of chromosomes (mechanism, cell biology, mitosis) |
H Ch. 6 (168-178) DNA replication |
|
| 9-07 | Meiosis, the basis of Mendel's laws and probability for genetics |
H Ch. 2 Mendel's breakthrough H Ch. 4 The chromosome theory, esp. 87-98. Binomial and Poisson distributions (also see Griffiths) |
|
| 9-12 | Genetic linkage and maps | H Ch. 5 Linkage and Mapping, esp. pp. 113-132 Chalfie 1997 (200 k. PDF) Supplemental text Hawley and Walker Ch. 7 |
|
| 9-14 | Linkage continued: |
H Ch. 5, esp. 132-145 tetrad analysis H Ch. 20, pp. 677-682 Hardy-Weinberg H Ch. 4 pp. 98-10 Validation of the chromosome theory (Bridges 1916) Hawley and Walker Ch. 7.3 & 7.4 |
|
| 9-19 | no class | ExonHit conference | |
| 9-21 | Genomics and Internet Resources |
H Ch. 10 Reconstructing the Genome |
Section II -- Methods of Molecular Biology
|
Date |
What |
Title |
Readings |
| 9-26 | Escherichia coli as a model organism and as a host for molecular cloning Homework 1 is due |
H Ch. 14 Genetic analysis in bacteria |
|
| 9-28 | no class | Mount has jury duty | |
| 10-03 | Lecture 8 |
Labeling, hybridization, microarrays and PCR Homework 2 is due |
H Ch. 9 DNA at high resolution, esp. pp. 297-310. H. Ch. 11. web sites |
| 10-05 | Lecture 9 |
Mutagenesis, sequencing and DNA-protein interactions Review for exam 1 |
H Ch. 9 pp. 297-310; H. Ch. 11 Direct detection of phenotype Fig. 16.15 on pg. 563. |
| 10-10 | EXAM | Exam on lectures 1-6 (Exam answers) | |
| 10-12 | Lecture 10 |
Genome organization: Genome structure and change | H. Ch. 10, pp. 339-363 H. Ch. 13 Chromosomal rearrangements H. Ch. 21 Molecular evolution Nature Genome Gateway (incl. Chimp) Fitch 2000 on homology (PDF, PubMed) |
| 10-17 | Lecture 11 |
Repair and recombination begin transcription |
H. Fig. 6-22; pp. 182-185; Ch. 7 Hartwell pp. 247-250; 581-586. Roeder 1996 Alberts online (transcription and repair). |
| 10-19 | Lecture 12 | Transcription Homework 3 is due |
H. Ch. 16 Gene regulation in Prokaryotes; |
Section III -- Molecular Genetics -- Gene Expression
|
Date |
What |
Title |
Readings |
| 10-24 | Regulation of transcription |
H Ch. 17,
especially 581-602 |
|
| 10-26 | Lecture 14 |
RNA Processing, translation, stability and localization |
H pp. 239-268 and 602-609 Nature: RNAi (esp. Matzke & Birchler) 2006 Nobel Prize in Medicine (Figure) |
| 10-31 | EXAM | This exam will cover lectures 7-12 ( Exam answers) | |
| 11-02 | Genetic and physical maps -- positional cloning |
H Ch. 5; Ch. 10, esp. 321-339; Ch. 11 Human Molecular Genetics II (online) |
|
| 11-07 | Lecture 16 (2006) |
LOD scores, complex traits and allelic association | H Ch. 5; Ch. 11; Ch. 20, esp. 692-699 Human Molecular Genetics II (online) Also see HAPMAP project in Nature (with commentary ) and HMGII (Chs. 11 and 12) |
| 11-09 | Lecture 17 (2006) |
Types of allele. | H. Chs. 3, 5, 7 (pp. 221-227), 11 (esp. 398-404) and 20 (esp. 692-699) |
Section IV -- Molecular Genetics -- Model Organisms and Genetic Analysis
|
Date |
What |
Title |
Readings |
| 11-14 | Lecture 18 (2006) |
Genome Projects and the relationships among species begin genetic analysis. Homework 4 is due |
H. Chs. 21, 19, and 10 (339-363) |
| 11-16 | Bioscience Day |
Bioscience Day (homework handed back ~11:10-11:15). Recommended and extremely relevant sessions are: 9:00: The Genomic Revolution: Comparative Analysis of Chimps, Humans & Dogs 11:30: Keynote speaker Eric Green: ""Sequence-based Exploration of the Human Genome: Circa 2006" 2:30: Genomics and the Future of Health Care |
No class due to Bioscience day. Homework 4 will be returned at 11:10-11:15. |
| 11-28 | EXAM | (review Exam answers) | review (.pdf) |
| 11-30 |
Lecture 19 (2006) |
Genetic analysis: Yeast (Saccharomyces cerevisiae) |
Hartwell Ch. 19, Ref. A |
| 12-05 | Lecture 20 (2006) |
C. elegans and D. melanogaster | Hartwell, Ref. C and Ref. D |
| 12-07 | Lecture 21 (2006) |
Arabidopsis thaliana and Mus musculus Genetic analysis with inbred lines |
Hartwell Ref. B and Ref. E HMG II online |
| 12-12 | Lecture 22 |
Genes in pathways: epistasis; review for final exam. Homework 5 is due | Hartwell 660-673 (esp. 660-665, pathways) |
| 12-14 | Final Exam |
Comprehensive final exam (one hour will be on section IV, lectures 18-23, and one hour will be comprehensive) | All readings! |