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LSM 510 Confocal Microscope
The Core Laboratory Facility's Zeiss LSM 510 confocal microscope has excitation laser lines at 458, 488, 543 and 633nm. It is has three separate detections channels and is capable of detecting many standard fluorophores, including CFP, GFP, fluorescein, Rhodamine, Texas Red, RFP, CY5 and several of the AlexFluor dyes. Suitable applications include imaging of single, double and triple-labeled specimens, 3-dimensional image analysis, colocalization studies, immunofluorescence, time series and FRAP experiments. A separate workstation with a copy of the Zeiss LSM software is available for image analysis and processing.
Faculty and students who wish to use the microscope should contact the facility manager to schedule a training session. Prepared slides will be used during the training session in order to acquaint new users with all aspects of the microscope, however, new users are also encouraged to bring their own samples to the training session if any are available. It is also highly recommended to review the UMD Quick-Start Guide before training begins.
The following rate schedule applies to different users. Fees are used to help cover the cost of the service contract on the microscope, which is partially subsidized by the department, and help pay for materials such as lens paper, lens cleaning solution and mercury bulbs. - Users within the College of Life Sciences: $10.00/hour
- On-campus users whose grants are not handled by the College of Life Sciences: $15.00/hour
- Users not affiliated with the campus: $30.00/hour
The microscope is available to trained users on an equal basis. An individual user may reserve one three hour block of time during peak hours and an additional 6 hours of time during off-peak hours.
Scheduling is accomplished using Oracle Calendar. New users who do not have an Oracle Calendar account will need to apply for a single account.
Cancellations made less than 24 hours in advance and any unused microscope time will be billed at 75% the standard rate for peak-usage times, or at 25% the standard rate for off-peak times.
All users are encouraged to familiarize themselves with the complete list of policies governing the use of the facility.
The facility manager is available during normal business hours to provide technical assistance to users. Additional resources include the following Visual Imaging Center protocols: Useful Links:
| Microscope | Axiovert100M (inverted) with universal stage insert available |
| Conventional Fluorescence Filters | Rhodamine, Fluorescein, LP505 |
| Lasers |
Argon (458 & 488nm), HeNe 543nm and HeNe 633nm |
| Objective Lenses | 10x/0.3, 40x/1.3 Oil DIC Plan NeoFluar, 63x/1.4 Oil DIC Plan Apo, 63x/1.2 W Korr C-Apo, 100x/1.4 Oil DIC Plan Apo |
| Dichroics | NT 80/20, HFT 488/543/633, HFT 488, HFT 458/543, HFT 488/543, HFT 458 |
| PMTs & Emission Filters | CH1 (LP 385,LP 475, LP 505, LP 530, LP 560, LP 650)
CH2 (LP 385, BP 385-470, BP 435-485, BP 475-525, BP 505-530, LP 505)
CH3 (LP 505, LP 560, BP 505-530, BP 505-550, BP 530-600, BP 560-615)
CHD (for transmitted light images) |
Amy Beaven
Manager
Core Laboratory Facility
0107 Microbiology Building
University of Maryland
abeaven@umd.edu
301-405-7238 |
