| We have developed the turnip crinkle virus (TCV) system as a model to study sequences and structures involved in replication and recombination of RNA templates and the interactions between the virus, host and parasitic subviral RNAs that lead to symptom production or host resistance. TCV (4054 bases) is among the smallest and simplest of the single component RNA viruses and is associated with numerous small, dispensable subviral RNAs (194 to 356 bases). Since subviral RNAs use the virus-encoded RNA-dependent RNA polymerase (RdRp) for replication, it is possible to use these simple, non-protein-coding RNAs as templates in replication and recombination studies. We have developed whole plant (turnip and Arabidopsis), protoplast (single plant cell) and in vitro systems with which to study the replication and recombination of viral genomic and subviral RNAs. In addition, subviral RNAs can modulate the symptoms of their helper viruses. Depending on the biological context, TCV subviral RNA satC can intensify or attenuate the symptoms of TCV on host plants. |
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